In situ analysis of endoplasmic reticulum stress-induced clustering of the endoplasmic reticulum stress sensor ERN1 and ligand-dependent β-arrestin recruitment to GPCRs illustrated the method's potential to study functional PPI modulation in complex cellular processes. We used KISS to evaluate interactions between different types of proteins, including transmembrane proteins, expressed at their native subcellular location. Benchmarking experiments indicate that in terms of sensitivity and specificity KISS is on par with other binary protein interaction technologies while being complementary with regard to the subset of PPIs it is able to detect. Here we present KISS, for KInase Substrate Sensor, a mammalian two-hybrid approach designed to map intracellular PPIs and some of the dynamic features they exhibit. (If you're interested in minimal design, Rob Pike's paper on a simple windowing system is a good read.Probably every cellular process is governed by protein-protein interaction (PPIs), which are often highly dynamic in nature being modulated by in- or external stimuli. This seems to stem with the authors' ( Russ Cox and Rob Pike come to mind) involvement with Plan9, which was a reimagination of UNIX with simplicity in mind. The term they use is "feature orthogonality", which means that any feature should only be added if it provides something truly unique. Go was designed to be simple (it's a decent read). However, I think the Go case is more interesting. ![]() With Lisp, all you have are functions and lists, which is about as simple as you can get (well, there's a little more, but whatever). ![]() When I think of minimialism, I think of Lisp and Go. In any case, I would be interested to learn about the designers' (documented) intentions rather than your personal opinion about a particular programming language. If you cite any specific language it would be great if you could add a link to some document in which this intent is clearly expressed by the language designers. to "allow an average programmer under average working conditions to write and maintain as much code as possible with the least cognitive effort"? So it is a commonly accepted idea that one should try to keep a piece of software simple (or simple stupid, in case you omit the comma) so that it easy to work on it later.īut can the KISS principle be applied also to programming language design? Do you know of any programming languages that have been designed specifically with this principle in mind, i.e. If I wanted to apply this to the field of software development I would replace "jet aircraft" with "piece of software", "average mechanic" with "average developer" and "under combat conditions" with "under the expected software development / maintenance conditions" (deadlines, time constraints, meetings / interruptions, available tools, and so on). ![]() Hence, the 'stupid' refers to the relationship between the way things break and the sophistication available to fix them. The principle is best exemplified by the story of Johnson handing a team of design engineers a handful of tools, with the challenge that the jet aircraft they were designing must be repairable by an average mechanic in the field under combat conditions with only these tools. ![]() here) is an important principle in software development, even though it apparently originated in engineering. KISS ("keep it simple, stupid" or "keep it simple stupid", see e.g.
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